2016 - 19th Annual Steven Galovich Memorial Student Symposium

Expanding the Application of Telomere-anchored PCR via Alteration of PCR conditions

Kojo Osafo, Lake Forest College

Abstract

Telomeres are protective, nucleoprotein complexes at chromosome termini. Since the length of telomeres has a role in cancer and aging, it is important to accurately measure the telomere DNA length. Our laboratory previously published a novel method to easily and inexpensively accomplish this in Aspergillus nidulans; however, we were unable to use this technique for organisms with other telomeric DNA sequences. I now have appropriately manipulated PCR conditions, such as using shorter primer permutations in combination with optimal annealing temperatures, to successfully measure the telomere length of another fungus, Aspergillus oryzae. A. oryzae has a much longer telomeric repeat than A. nidulans, which suggests that this technique could be used by other researchers to measure the telomere length in organisms having a wide variety of telomere sequences.

 
Apr 5th, 10:30 AM Apr 5th, 11:45 AM

Expanding the Application of Telomere-anchored PCR via Alteration of PCR conditions

Mohr Walkway

Telomeres are protective, nucleoprotein complexes at chromosome termini. Since the length of telomeres has a role in cancer and aging, it is important to accurately measure the telomere DNA length. Our laboratory previously published a novel method to easily and inexpensively accomplish this in Aspergillus nidulans; however, we were unable to use this technique for organisms with other telomeric DNA sequences. I now have appropriately manipulated PCR conditions, such as using shorter primer permutations in combination with optimal annealing temperatures, to successfully measure the telomere length of another fungus, Aspergillus oryzae. A. oryzae has a much longer telomeric repeat than A. nidulans, which suggests that this technique could be used by other researchers to measure the telomere length in organisms having a wide variety of telomere sequences.